Elaborating Of Effective Preparates With Immunomodulating Properties On
The Base Of Transfer Factor Of Cell-Mediated Immune Response
Authors: T.A. Lyubchenko, E.G. Goleva, L.S. Kholodna, V.V. Smirnov
Institution: Faculty Of Biology Dept. Of Microbiology And Immunology, Ukraine
Abstract:
Transfer Factor – a cytokine derived from dialyzable leukocyte extracts
(DLE), is capable to transfer and induce antigenspecific cell-mediated
immune response. The purpose of this work was biotechnological production,
purification and immunological testing of human and animal Transfer Factors
(TF). As a result it is planned to use Transfer Factor as a therapeutical
drug in Ukraine. Staphylococcus aureus Cowan-1 (2352) and Wood-46 (2351),
staphylococcal cell-bounded protein A, staphylococcal anatoxin and diphteria
anatoxin were used as antigens. TF was obtained from DLE of human donors
and calves, that had delayed type hypersensitivity (DTH) to above metioned
anigen substances. Extracts were purified by gel-filtration on Sephadex
G-25. Substances with activity of TF was found in fraction with molecular
weight of 5-10kD. By means of high voltage electrophoresis on paper it
was shown that this substance consists of protein and nuclear acid parts.
Obviously the protein part of TF cause its immunological effects. Preparates
were not harmful, pirogenic, toxic and did not cause allergy; HIV antigens
and antigens of hepatitis B were not present in them. Mutagenic effects
were not found. TF to Staphylococcus aureus antigen substances was antigenspecific,
TF to diphteria anatoxin possessed also adjuvant properties. All preparates
transferred the DTH reactivity from immunized organism to nonimmune, induced
the production of inhibition migration factor, IL-1, IL-2, showed interspecial
activity. Immunomodulating effects of TF on cellular immunity and antibody
production were shown. It was tried to administrate of Transfer Factor
to mice of Balb/c strain with experimental Staphylococcal infection. The
effective doses and routes of administration had been worked out.