Functional Activity Of Lymphocytes In Rats With Radiation Induced Hypothyreosis
After Xenotransplantation Of Thyroid Gland Organ Culture And Immunostimulation
With Transfer Factor
Authors: E.G, Goleva., Lyubchenko T.A., Pasteur I.P.,*, Kholodna L.S.,
Gorban E.N.*, Donich S.F.*, Tronko M.D.*
Institution: Faculty Of Biology Dept. Of Microbiology And Immunology, Ukraine
Abstract:
The purpose of this investigation was to study the functional activity
of lymphocytes from rats with radiation-induced hypothyreosis after xenotransplantation
of newborn pig thyroid gland organ culture and immunostimulating influence
of transfer factor (purified and fractionated dialyzable leukocyte extract)
on it. Such transplantation didn´t reduce immune activity of rat´s
splenocytes in vitro. Preparations of human and animal transfer factor
ativated (3H)-thymidine incorporation into splenocyte´s DNA, enhanced
activity of macrophages and their antigen-presenting functions in intact
animals as well as in rats with thyroid gland xenotransplantat. There was
also group of rats with irradiated thyroid gland, which function was substituted
by injections of (1311). In this group we observed reducing of lymphocytes
functional activity, which was successfully corrected with transfer factor.
We concluded that xenotransplantation of thyroid gland organ culture didn’t
reduce immune activity of recipient´s splenocytes and macrophages.
Transfer factor can be used as a stimulator for immunodeficiency caused
by thyroid gland disfunctions.